My bacteria plate has been kept in my uncle's office since September 16th. Each day, a picture was taken to monitor growth of bacteria in Nutrient Broth. The photos were all taken at approximately 5:00 PM before being exposed for 20 minutes in a room with a fixed temperature of 72-75 degrees Fahrenheit (or about 22-24 degrees Celsius).
Results A: Area of Colonies Per DayResults B [Table]: Types of Colonies Per Day | Orange | Black | Yellow | White | Green | Total | Day 1 | 0 | 0 | 0 | 0 | 0 | 0 | Day 2 | 0 | 0 | 0 | 0 | 0 | 0 | Day 3
| 0 | 0 | 0 | 0 | 0 | 0 | Day 4 | 0 | 0 | 3 | 7 | 1 | 11 | Day 5 | 2 | 0 | 4 | 11 | 2 | 19 | Day 6 | 3 | 0 | 4 | 17 | 4 | 28 | Day 7 | 4 | 0 | 7 | 17 | 5 | 33 | Day 8 | 6 | 1 | 10 | 17 | 4 | 38 | Day 9 | 6 | 1 | 15 | 10 | 8 | 40 | Day 10 | 5 | 1 | 13 | 12 | 14 | 45 | Day 11 | 5 | 1 | 15 | 9 | 18 | 48 | Day 12 | 7 | 1 | 12 | 10 | 20 | 50 | Day 13 | 6 | 1 | 14 | 12 | 17 | 50 | Day 14 | 5 | 1 | 10 | 7 | 10 | 33 | Day 15 | 5 | 1 | 12 | 8 | 14 | 40 |
Results B [Graph]: Types of Colonies Per Day [Graph 2] Total Colonies Per Day
Conclusion: My bacteria plate was exposed for 20 minutes each day for 15 days to the atmosphere in my uncle's office. Around day 4, bacteria started growing steadily, but then after the total number of colonies grew to 50 the population became more unstable. This is an example of Logistic Growth, with a carrying capacity of approximately 50. The entire plate had not been covered, and I believe this is because there are limited resources in the plate. There was not enough agar to sustain a population of greater than 50. None of the colonies grew to be very big and were all spread out. I think this is because each of the bacteria colonies required a relatively large amount of agar, and absorbed some of the nutrients from the area around the colony. The colonies had to spread out in order to find a spot with enough agar to grow. What I Learned: During this project, I had a chance to observe logistic growth in a 'real' situation. I learned that the properties of carrying capacity and logistic growth apply to bacteria and fungi. The bacteria plate had a limited amount of space and nutrients, so they could not grow exponentially. With a larger plate and more agar, there would be a greater potential for more colonies. |
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